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<h1 id="firstHeading" class="firstHeading mw-first-heading"><span class="mw-page-title-main">CLIP-Seq</span></h1>
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<div id="mw-content-text" class="mw-body-content mw-content-ltr" lang="de" dir="ltr"><div class="mw-content-ltr mw-parser-output" lang="de" dir="ltr"><p><b>CLIP-Seq</b> (engl. <span lang="en"><i>cross-linking immunoprecipitation-high-throughput sequencing</i>, ‚Quervernetzung und Immunpräzipitation mit Sequenzierung im Hochdurchsatz‘</span>) ist eine Methode der <a href="Biochemie" title="Biochemie">Biochemie</a> zur Untersuchung von <a href="Protein-RNA-Interaktion" title="Protein-RNA-Interaktion">RNA-Protein-</a> und RNA-RNA-Interaktionen.<sup id="cite_ref-1" class="reference"><a href="#cite_note-1"><span class="cite-bracket">[</span>1<span class="cite-bracket">]</span></a></sup> Gelegentlich wird die CLIP-Seq auch als <i>HITS-CLIP</i> bezeichnet.<sup id="cite_ref-2" class="reference"><a href="#cite_note-2"><span class="cite-bracket">[</span>2<span class="cite-bracket">]</span></a></sup><sup id="cite_ref-3" class="reference"><a href="#cite_note-3"><span class="cite-bracket">[</span>3<span class="cite-bracket">]</span></a></sup><sup id="cite_ref-4" class="reference"><a href="#cite_note-4"><span class="cite-bracket">[</span>4<span class="cite-bracket">]</span></a></sup>
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<div class="mw-heading mw-heading2"><h2 id="Prinzip">Prinzip</h2></div>
<p>Die CLIP-Seq besteht aus einer Kombination einer <a href="Ultraviolettes_Licht" class="mw-redirect" title="Ultraviolettes Licht">UV</a>-<a href="Vernetzung_(Chemie)" title="Vernetzung (Chemie)">Quervernetzung</a>, einer <a href="Immunpr%C3%A4zipitation" title="Immunpräzipitation">Immunpräzipitation</a>, einer teilweisen <a href="RNase" class="mw-redirect" title="RNase">Nukleolyse</a> und einer <a href="DNA-Sequenzierung" title="DNA-Sequenzierung">DNA-Sequenzierung</a> im <a href="Hochdurchsatz" class="mw-redirect" title="Hochdurchsatz">Hochdurchsatz</a>.
</p><p>Die CLIP-Seq besitzt Ähnlichkeiten zum <a href="PAR-CLIP" title="PAR-CLIP">PAR-CLIP</a>, zum <a href="ICLIP" title="ICLIP">iCLIP</a>, zum <a href="RIP-Chip" title="RIP-Chip">RIP-Chip</a> (ohne Vernetzung und mit <a href="Microarray" title="Microarray">Microarray</a> anstatt der DNA-Sequenzierung), zur <a href="SELEX" title="SELEX">SELEX</a>, zur <a href="ChIP-Seq" title="ChIP-Seq">ChIP-Seq</a> (zur Untersuchung von <a href="DNA" class="mw-redirect" title="DNA">DNA</a>-Protein-Interaktionen) und zum <a href="ChIP-on-Chip" title="ChIP-on-Chip">ChIP-on-Chip</a> (zur Untersuchung von DNA-Protein-Interaktionen mit einem Microarray anstatt der DNA-Sequenzierung).
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<div class="mw-heading mw-heading2"><h2 id="Weblinks">Weblinks</h2></div>
<ul><li><a rel="nofollow" class="external text" href="https://starbase.sysu.edu.cn/">starBase database</a>: eine Online-Datenbank für RNA-Protein-Interaktionen. Abgerufen am 20. Juni 2013.</li>
<li><a rel="nofollow" class="external text" href="https://dorina.mdc-berlin.de/">BIMSB doRiNA database</a>: eine Online-Datenbank für RNA-Protein-Interaktionen. Abgerufen am 20. Juni 2013.</li></ul>
<div class="mw-heading mw-heading2"><h2 id="Einzelnachweise">Einzelnachweise</h2></div>
<ol class="references">
<li id="cite_note-1"><span class="mw-cite-backlink"><a href="#cite_ref-1">↑</a></span> <span class="reference-text">Sanford JR, Wang X, Mort M, <i>et al.</i>: <cite style="font-style:italic">Splicing factor SFRS1 recognizes a functionally diverse landscape of RNA transcripts</cite>. In: <cite style="font-style:italic">Genome Res.</cite> 19. Jahrgang, <span style="white-space:nowrap">Nr.<span style="display:inline-block;width:.2em"> </span>3</span>, März 2009, <span style="white-space:nowrap">S.<span style="display:inline-block;width:.2em"> </span>381–94</span>, <a href="Digital_Object_Identifier" title="Digital Object Identifier">doi</a>:<span class="uri-handle" style="white-space:nowrap"><a rel="nofollow" class="external text" href="https://doi.org/10.1101/gr.082503.108">10.1101/gr.082503.108</a></span>, <a class="external mw-magiclink-pmid" rel="nofollow" href="https://www.ncbi.nlm.nih.gov/pubmed/19116412?dopt=Abstract">PMID 19116412</a>, <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2661799/">PMC 2661799</a> (freier Volltext).<span class="Z3988" title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rfr_id=info:sid/de.wikipedia.org:CLIP-Seq&rft.atitle=Splicing+factor+SFRS1+recognizes+a+functionally+diverse+landscape+of+RNA+transcripts&rft.au=Sanford+JR%2C+Wang+X%2C+Mort+M%2C+...&rft.date=2009-03&rft.doi=10.1101%2Fgr.082503.108&rft.genre=journal&rft.issue=3&rft.jtitle=Genome+Res.&rft.pages=381-94&rft.pmc=2661799&rft.pmid=19116412&rft.volume=19.+Jahrgang" style="display:none"> </span></span>
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<li id="cite_note-2"><span class="mw-cite-backlink"><a href="#cite_ref-2">↑</a></span> <span class="reference-text">Darnell RB: <cite style="font-style:italic">HITS-CLIP: panoramic views of protein-RNA regulation in living cells</cite>. In: <cite style="font-style:italic">Wiley Interdiscip Rev RNA.</cite> 1. Jahrgang, 2010, <span style="white-space:nowrap">S.<span style="display:inline-block;width:.2em"> </span>266–86</span>, <a href="Digital_Object_Identifier" title="Digital Object Identifier">doi</a>:<span class="uri-handle" style="white-space:nowrap"><a rel="nofollow" class="external text" href="https://doi.org/10.1002/wrna.31">10.1002/wrna.31</a></span>.<span class="Z3988" title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Abook&rfr_id=info:sid/de.wikipedia.org:CLIP-Seq&rft.atitle=HITS-CLIP%3A+panoramic+views+of+protein-RNA+regulation+in+living+cells&rft.au=Darnell+RB&rft.btitle=Wiley+Interdiscip+Rev+RNA.&rft.date=2010&rft.doi=10.1002%2Fwrna.31&rft.genre=book&rft.pages=266-86&rft.volume=1.+Jahrgang" style="display:none"> </span></span>
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<li id="cite_note-3"><span class="mw-cite-backlink"><a href="#cite_ref-3">↑</a></span> <span class="reference-text">Licatalosi DD, Mele A, Fak JJ, <i>et al.</i>: <cite style="font-style:italic">HITS-CLIP yields genome-wide insights into brain alternative RNA processing</cite>. In: <cite style="font-style:italic">Nature</cite>. 456. Jahrgang, <span style="white-space:nowrap">Nr.<span style="display:inline-block;width:.2em"> </span>7221</span>, November 2008, <span style="white-space:nowrap">S.<span style="display:inline-block;width:.2em"> </span>464–9</span>, <a href="Digital_Object_Identifier" title="Digital Object Identifier">doi</a>:<span class="uri-handle" style="white-space:nowrap"><a rel="nofollow" class="external text" href="https://doi.org/10.1038/nature07488">10.1038/nature07488</a></span>, <a class="external mw-magiclink-pmid" rel="nofollow" href="https://www.ncbi.nlm.nih.gov/pubmed/18978773?dopt=Abstract">PMID 18978773</a>, <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2597294/">PMC 2597294</a> (freier Volltext).<span class="Z3988" title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rfr_id=info:sid/de.wikipedia.org:CLIP-Seq&rft.atitle=HITS-CLIP+yields+genome-wide+insights+into+brain+alternative+RNA+processing&rft.au=Licatalosi+DD%2C+Mele+A%2C+Fak+JJ%2C+...&rft.date=2008-11&rft.doi=10.1038%2Fnature07488&rft.genre=journal&rft.issue=7221&rft.jtitle=Nature&rft.pages=464-9&rft.pmc=2597294&rft.pmid=18978773&rft.volume=456.+Jahrgang" style="display:none"> </span></span>
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<li id="cite_note-4"><span class="mw-cite-backlink"><a href="#cite_ref-4">↑</a></span> <span class="reference-text">Ingolia NT, Ghaemmaghami S, Newman JR, Weissman JS: <cite style="font-style:italic">Genome-wide analysis in vivo of translation with nucleotide resolution using ribosome profiling</cite>. In: <cite style="font-style:italic">Science</cite>. 324. Jahrgang, <span style="white-space:nowrap">Nr.<span style="display:inline-block;width:.2em"> </span>5924</span>, April 2009, <span style="white-space:nowrap">S.<span style="display:inline-block;width:.2em"> </span>218–23</span>, <a href="Digital_Object_Identifier" title="Digital Object Identifier">doi</a>:<span class="uri-handle" style="white-space:nowrap"><a rel="nofollow" class="external text" href="https://doi.org/10.1126/science.1168978">10.1126/science.1168978</a></span>, <a class="external mw-magiclink-pmid" rel="nofollow" href="https://www.ncbi.nlm.nih.gov/pubmed/19213877?dopt=Abstract">PMID 19213877</a>, <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2746483/">PMC 2746483</a> (freier Volltext).<span class="Z3988" title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rfr_id=info:sid/de.wikipedia.org:CLIP-Seq&rft.atitle=Genome-wide+analysis+in+vivo+of+translation+with+nucleotide+resolution+using+ribosome+profiling&rft.au=Ingolia+NT%2C+Ghaemmaghami+S%2C+Newman+JR%2C+...&rft.date=2009-04&rft.doi=10.1126%2Fscience.1168978&rft.genre=journal&rft.issue=5924&rft.jtitle=Science&rft.pages=218-23&rft.pmc=2746483&rft.pmid=19213877&rft.volume=324.+Jahrgang" style="display:none"> </span></span>
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